paired end sequencing wikipedia
Sequencing technologies vary in the length of reads produced. Read length is a factor which can affect the results of biological studies.
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The chain-termination method of DNA sequencing Sanger sequencing can only be used for short DNA strands of 100 to 1000 base pairsDue to this size limit longer sequences are subdivided.
. It is named by analogy with the rapidly expanding quasi-random shot grouping of a shotgun. Typical sequencers produce read lengths in the range of 100-500 bp. Reads of length 20-40 base pairs bp are referred to as ultra-short.
However Pacific Biosciences platforms produce read lengths of approximately 1500 bp. In genetics shotgun sequencing is a method used for sequencing random DNA strands.
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